adding kozak sequence to primer

Azam Rahimpour via methods%40net.bio.net (by rahimpour_a from yahoo.com)
Tue Oct 27 09:28:41 EST 2009


Hi
I want to add kozak sequence to my primer for mamamlian expression but I know the consensus kozak sequence is gccgcc(a/g)ccAUGg but the second codon in my gene is started with a not g. should I convert it to g  to make it identical to kozak or using just gccgcc(a/g)ccAUG is OK?
best


      


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