Custom ISH probe synthesis
Arne Christensen
via methods%40net.bio.net
(by arnec from bio.umass.edu)
Tue Oct 27 12:09:23 EST 2009
Dear Listeners,
I am in a lab that does a great deal of protein work, but we have
limited experience conducting molecular methods such as subcloning and
ISH. We would like to run some ISH, and we are prepared to purchase the
reagents to do so, however we are not in a position to set ourselves up
for the molecular work underlying the generation of a plasmid for making
RNA probes. So, we would like to purchase four RNA in situ probes. Two
target probes with their respective sense negative controls. The
sequences are very short, a 174-mer and a 71-mer. Can anybody
recommend a commercial source for such RNA probe synthesis?
Thank you in advance,
Arne
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