Inclusion bodies centrifugation

megha goyal via methods%40net.bio.net (by mgbiotec from gmail.com)
Tue Oct 27 22:18:33 EST 2009


Dear All,

Our protein is expressed as inclusion bodies and I want to separate
inclusion bodies from E.coli from the cellular debris after* *lysis of the
cells by sonication.

Can I do this by normal centrifugation? and if yes, at what speed?

Our centrifuge has maximum speed of 14000 rpm. Can we do the separation
using this centrifuge and if so how.



Thanking in anticipation.


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