cellular localization of L-arginine on confocal

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Tue Sep 8 08:50:41 EST 2009


Am 04.09.2009, 12:53 Uhr, schrieb Emerson Martins <martinseac from gmail.com>:

> Hi All
> I have to localize L-arginine in live cells on confocal microscopy. Does
> anyone know some fluorescent marker that I can use to treat cells to see
> L-arginine influx?

Arg can be labelled with reagents that carry a glyoxal-group (-CO-CO-).  
However, that's usually done with peptidyl-Arg, not the free amino acid.  
How is the Arg supposed to enter the cell? Can you use patch clamp or  
potential sensitive dyes?


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