cellular localization of L-arginine on confocal
Emerson A. Castilho Martins
(by martinseac from gmail.com)
Tue Sep 8 13:44:00 EST 2009
That's the problem. I want to label free L-arginine. I can use
H3-L-arginine* to this, but I don't know a method to detect this
accumulation by confocal microscopy, just by scintillography. Arg will
enter the cell by cationic amino acids transporters, that was already
Em Tue, 08 Sep 2009 10:50:41 -0300, Dr Engelbert Buxbaum
<engelbert_buxbaum from hotmail.com> escreveu:
> Am 04.09.2009, 12:53 Uhr, schrieb Emerson Martins <martinseac from gmail.com>:
>> Hi All
>> I have to localize L-arginine in live cells on confocal microscopy. Does
>> anyone know some fluorescent marker that I can use to treat cells to see
>> L-arginine influx?
> Arg can be labelled with reagents that carry a glyoxal-group (-CO-CO-).
> However, that's usually done with peptidyl-Arg, not the free amino acid.
> How is the Arg supposed to enter the cell? Can you use patch clamp or
> potential sensitive dyes?
> Methods mailing list
> Methods from net.bio.net
Usando o revolucionário cliente de e-mail do Opera:
More information about the Methods