RNAseH

Van der Merwe, M, Me <mathilde from sun.ac.za> via methods%40net.bio.net (by mathilde from sun.ac.za)
Wed Sep 9 07:39:45 EST 2009


Hi all

I am doing reverse transcription with Superscript II and oligo(dT)s as the first step of a 2-step RT qPCR. I will do relative quantification on fragments  not exceeding 250 bp in size.

How necessary is it to RNAseH treat my cDNA/RNA after reverse transcription? If I don't, will it cause trouble downstream?

Thanks!
Mathilde




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