RNAseH

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Wed Sep 9 12:39:07 EST 2009


On Sep 9, 7:39 am, "Van der Merwe, M, Me <mathi... from sun.ac.za>"
<mathi... from sun.ac.za> wrote:
> Hi all
>
> I am doing reverse transcription with Superscript II and oligo(dT)s as the first step of a 2-step RT qPCR. I will do relative quantification on fragments  not exceeding 250 bp in size.
>
> How necessary is it to RNAseH treat my cDNA/RNA after reverse transcription? If I don't, will it cause trouble downstream?
>
> Thanks!
> Mathilde

I never do RNase H treatment for routine quantitative or semi-
quantitative RT-PCR.

Nick

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