pTopo purification problems

Yoram Gerchman via methods%40net.bio.net (by gerchman from research.haifa.ac.il)
Thu Sep 10 14:54:58 EST 2009


Greetings netters
I have recently made an attempt to clone a short gene into pTopo (using Top10
competent cells). i did get white colonies on LB-Amp-X-gal, and they grew
nicely on liquid LB-Amp, but after plasmid purification I get no DNA (tried
already 2 different kits). Any ideas as to how remediate this situation?
Many thanks
Yoram



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