protease cleavage site program

Yoram Gerchman via methods%40net.bio.net (by gerchman from research.haifa.ac.il)
Wed Apr 7 01:04:28 EST 2010


Dear Cristina
Expasy <www.Expasy.ch> is a great site for DNA, RNA and protein related tools.
For your specific question try <http://www.expasy.ch/tools/peptidecutter/>.
Yoram


>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 6 Apr 2010 02:02:42 -0700 (PDT)
> From: LeboMad <lmadubanya from gmail.com>
> Subject: PCR failing
> To: methods from net.bio.net
> Message-ID:
> 	<5a4d0356-fa5d-45ea-8b98-4b1eed90bc72 from u31g2000yqb.googlegroups.com>
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>
> Hi all. I have a serious problem with PCR amplification in my lab. I
> have done all troubleshooting there is out there I can't seem to get
> it right. I normally get nice clear bands but for some reason I get
> very faint bands if any at all. Can anyone suggest anything. I've got
> a student that needs some PCR runs and needs result as in yesterday.
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 06 Apr 2010 13:05:32 +0000
> From: Christian Praetorius <prae from gmx.net>
> Subject: Re: PCR failing
> To: methods from net.bio.net
> Message-ID: <820psuFd11U1 from mid.individual.net>
> Content-Type: text/plain; charset=us-ascii
>
> LeboMad <lmadubanya from gmail.com> wrote:
>
> >have done all troubleshooting there is out there I can't seem to get
> >it right. I normally get nice clear bands but for some reason I get
> >very faint bands if any at all. Can anyone suggest anything. I've got
> >a student that needs some PCR runs and needs result as in yesterday.
>
> The nyou should describing *exactly* what your procedure is and what
> you tried to troubleshoot.
>
> Christian
>
> --
> X-no-Sig: yes
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 6 Apr 2010 15:44:52 +0200
> From: Cristina Echevarr?a Ruiz de Vargas <echeva from us.es>
> Subject: protease cleavage site program
> To: <methods from magpie.bio.indiana.edu>
> Message-ID: <EAF924AB084346398E5D96492E21F756 from PRMD2>
> Content-Type: text/plain;	charset="iso-8859-1"
>
> Dear colegue,
> I has sow your e-mail direction associated with a program to evaluated
> possibles cleavege sites in a protein. I have try to use this information but
> it was not possible probably because  I dont know. So I will send you a
> peptide ERHHSIDAQLRALAPGKVSEE  and I would ask you the possibility to look a
> possible cleavege site in this peptides. Sorry for my Inglish. A would
> apreciated very much if you can loock for this as I am working with a
> protease that seem to attack this peptide.
>
> Tahnk you very much in advance
>
> Cristina Echevarría
>
> *****************************************************
> Cristina Echevarría
> Profesora Titular  de Universidad
> Dpto. Biología Vegetla y Ecología
> Facultad de Biología
> Universidad de Sevilla
> Sevilla
> España
>
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> End of Methods Digest, Vol 59, Issue 3
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