Long Ranger Gel - Bad polymerization

[Alex - Exon Biotecnologia]

Hi all,

I am used to prepare Long Ranger gel solutions to run DNA small fragments
(microsatellites) in a ABI 377 sequencer.

Since the beggining of the week, I have had problems with the polymerization
of the gel even using the same reagents I used before. We tried to change
all the reagents, but this didn't work. Normally, we wait 1:30 hour to load
the gel and start the run.

Does anybody know what might be the problem? It's very cold here since
sunday, but I don't think this can be the reason of the problem...

Thanks,


Alex