PCR failing
Duncan Clark
via methods%40net.bio.net
(by blackhole from abuse.plus.com)
Fri Apr 9 03:13:24 EST 2010
Historians believe that in newspost
<9de680cc-d2fe-478c-ba5d-bfbc2b53b763 from u31g2000yqb.googlegroups.com> on
Thu, 8 Apr 2010, LeboMad <lmadubanya from gmail.com> penned the following
literary masterpiece:
>2mM dNTP
0.2mM surely? 2mM would chelate out all the Mg and it would never work.
I assume each PCR product amplifies fine in a non-multiplex and only
when you multiplex there is a problem?
Primer levels used?
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
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