PCR failing

Duncan Clark via methods%40net.bio.net (by blackhole from abuse.plus.com)
Fri Apr 9 03:13:24 EST 2010


Historians believe that in newspost 
<9de680cc-d2fe-478c-ba5d-bfbc2b53b763 from u31g2000yqb.googlegroups.com> on 
Thu, 8 Apr 2010, LeboMad <lmadubanya from gmail.com> penned the following 
literary masterpiece:
>2mM dNTP

0.2mM surely? 2mM would chelate out all the Mg and it would never work.

I assume each PCR product amplifies fine in a non-multiplex and only 
when you multiplex there is a problem?

Primer levels used?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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