MSAP

Parivartan vishal via methods%40net.bio.net (by parivishal from gmail.com)
Mon Apr 12 01:45:28 EST 2010


hello everyone,

i am encountring problem in Methylation Sensitive Amplified Polymorphism .
i  restricted DNA with 2 isoshizomers *i.e* MspI and HpaII separately along
with EcoRI  as the common enzyme. reestriction is OK. but adaptors are not
being ligated successfully to the restricted fragments.
what i see is a band ahead of the dye which i probably think is the
unligated adaptors. and if u cud plz suggest a protocol for the preparation
of adaptors

-- 
Parivartan Vishal
School of Biotechnology
Univ. of Jammu
Jammu
INDIA


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