(by parivishal from gmail.com)
Mon Apr 12 01:45:28 EST 2010
i am encountring problem in Methylation Sensitive Amplified Polymorphism .
i restricted DNA with 2 isoshizomers *i.e* MspI and HpaII separately along
with EcoRI as the common enzyme. reestriction is OK. but adaptors are not
being ligated successfully to the restricted fragments.
what i see is a band ahead of the dye which i probably think is the
unligated adaptors. and if u cud plz suggest a protocol for the preparation
School of Biotechnology
Univ. of Jammu
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