Antigen Retrieval Problems
(by yvonne.couch from pharm.ox.ac.uk)
Mon Apr 12 11:47:57 EST 2010
I'm trying to get an awful antibody to work on some tissue sections. It's
currently a 3-night stain in the fridge and I'm trying to shorted the
procedure. I've tried citrate buffer based antigen retrieval but its on
frozen PFA fixed sections and I suspect the gelatine coating is not as good
as it could be since they're all floating off the slides in the microwave.
Anyone got any other suggestions?
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