Denaturation of Streptavidin-Biotin bound dsDNA into ssDNA

Guo Wei Kua via methods%40net.bio.net (by gwkboy from gmail.com)
Mon Apr 12 21:47:52 EST 2010


Hi,

Thank you all for your help. I have followed the ammonium hydroxide way. I
do get ssDNA, but alot of them are actually the primers itself. Currently, I
am removing the primers using MWCO columns, then do the bead binding again,
I see whether it will help. Will update =D


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