why do we only use γ-[32P]ATP to radiolable RNA?

[Raymond Dalgleish]

Ruiying Wang wrote:
> Hi,
> 
> I have been using γ-[32P]ATP to radiolable my RNA, but I can not find the
> reason why we only use γ-[32P]ATP to radiolable RNA, why do not use other
> γ-[32P]NTPs?
> 
> Thanks,
> 
> Ruiying
If you are labelling using γ-[32P]ATP it implies that you are doing so 
using polynucletide kinase to label 5' ends. Only ATP can act as a donor 
of the radioactive phosphate group.

Hope that helps.

Raymond Dalgleish