why do we only use γ-[32P]ATP to radiolable RNA?

Raymond Dalgleish via methods%40net.bio.net (by rwmd1 from le.ac.uk)
Wed Apr 14 05:46:34 EST 2010


Ruiying Wang wrote:
> Hi,
> 
> I have been using γ-[32P]ATP to radiolable my RNA, but I can not find the
> reason why we only use γ-[32P]ATP to radiolable RNA, why do not use other
> γ-[32P]NTPs?
> 
> Thanks,
> 
> Ruiying
If you are labelling using γ-[32P]ATP it implies that you are doing so 
using polynucletide kinase to label 5' ends. Only ATP can act as a donor 
of the radioactive phosphate group.

Hope that helps.

Raymond Dalgleish


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