why do we only use γ-[32P]ATP to radiolable RNA?
(by rwmd1 from le.ac.uk)
Wed Apr 14 05:46:34 EST 2010
Ruiying Wang wrote:
> I have been using γ-[32P]ATP to radiolable my RNA, but I can not find the
> reason why we only use γ-[32P]ATP to radiolable RNA, why do not use other
If you are labelling using γ-[32P]ATP it implies that you are doing so
using polynucletide kinase to label 5' ends. Only ATP can act as a donor
of the radioactive phosphate group.
Hope that helps.
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