Methods Digest, Vol 59, Issue 11 - RNA probes

Priscila Peña Diaz via methods%40net.bio.net (by ofiura from gmail.com)
Wed Apr 14 13:44:12 EST 2010


oops! RNA probes, my bad.....
I talked about *DNA probes* for detecting RNA. Apologies for the
misunderstanding

P.


> Hi,
> >
> > I have been using ã-[32P]ATP to radiolable my RNA, but I can not find the
> > reason why we only use ã-[32P]ATP to radiolable RNA, why do not use other
> > ã-[32P]NTPs?
> >
> > Thanks,
> >
> > Ruiying
>
>
>
> Hello
>
>
> We make RNA probes using PCR with [32P]dCTP in the mix for labelling.  So
> far it has worked great, always.  This is actually the only dNTP we use for
> the labelling, but I don't discard its posible to use others.
>
> P.
>
> > ------------------------------
> >
> >
> >
>
>
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>
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> End of Methods Digest, Vol 59, Issue 11
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