NUpage gel problem

Lori Long via methods%40net.bio.net (by LLong from kbpllc.com)
Wed Apr 21 08:06:38 EST 2010


I would check the level of your buffer before you run.  Make sure it is right up to the rim and then watch to make sure the level doesn't drop, it is probably leaking.  Fill your inner chamber first and watch it before filling the outer chamber. 

Lori Long
Quality Control Manager
Kentucky BioProcessing, LLC
3700 Airpark Drive  Owensboro, KY  42301
ph 270-663-6132   fax 270-689-2571

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of ZHOU Jie
Sent: Wednesday, April 21, 2010 12:40 AM
To: 'Nick Theodorakis'; methods from magpie.bio.indiana.edu
Subject: RE: NUpage gel problem

Have you removed the white seal at the bottom of the gel?

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Nick Theodorakis
Sent: 2010年4月21日 1:27
To: methods from magpie.bio.indiana.edu
Subject: Re: NUpage gel problem

On Apr 20, 4:58 am, Chris McDermott-Roe
<cjmcdermott... from googlemail.com> wrote:
> Hi all,
>
> I'm having a rather basic and embarassing problems with an Invitrogen NuPAGE
> gel.  I'm attempting to run a regular bis-tris 4-12 % bis-tris pre-cast at
> 100 V using the supplied 20x MOPS SDS buffer but literally a second or two
> after commencing, all stops and an E1 message appears on my power supply.
> The only way I can actually get my proteins to go anywhere is by replacing
> the dummy gel which I've put in the tank with a second real gel.  I'm v
> puzzled and any help is gratefully received.


Is the buffer leaking out when a dummy gel is attached? Perhaps the
"real gels" form a better seal on the tank than the dummy.

Nick

--
Nick Theodorakis
nick_theodorakis from hotmail.com
contact form:
http://theodorakis.net/contact.html
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