NUpage gel problem

[Taliaferro, Dwayne (NIH/NIMH) [F]]

Your gel running apparatus could be failing.  Try another one.

On 4/21/10 1:39 AM, "ZHOU Jie" <jetjie from ust.hk> wrote:

Have you removed the white seal at the bottom of the gel?

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of Nick Theodorakis
Sent: 2010年4月21日 1:27
To: methods from magpie.bio.indiana.edu
Subject: Re: NUpage gel problem

On Apr 20, 4:58 am, Chris McDermott-Roe
<cjmcdermott... from googlemail.com> wrote:
> Hi all,
>
> I'm having a rather basic and embarassing problems with an Invitrogen NuPAGE
> gel.  I'm attempting to run a regular bis-tris 4-12 % bis-tris pre-cast at
> 100 V using the supplied 20x MOPS SDS buffer but literally a second or two
> after commencing, all stops and an E1 message appears on my power supply.
> The only way I can actually get my proteins to go anywhere is by replacing
> the dummy gel which I've put in the tank with a second real gel.  I'm v
> puzzled and any help is gratefully received.


Is the buffer leaking out when a dummy gel is attached? Perhaps the
"real gels" form a better seal on the tank than the dummy.

Nick

--
Nick Theodorakis
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