NUpage gel problem
(by stewjw from gmail.com)
Thu Apr 22 03:39:25 EST 2010
When setiing up fill the upper buffer chamber first with around 200ml
buffer and check for leaks before filling the lower chamber (600ml).
Replace the XCell Surelock gaskets if they are leaking , Invitrogen
The recommended voltage for NUPAGE Bis-Tris gels is 200V or 150-175V
for 9 and 17 well gels.
Some good advice in the Manual:
More information about the Methods