NUpage gel problem

StewJW via methods%40net.bio.net (by stewjw from gmail.com)
Thu Apr 22 03:39:25 EST 2010


When setiing up fill the upper buffer chamber first with around 200ml
buffer and check for leaks before filling the lower chamber (600ml).
Replace the XCell Surelock gaskets if they are leaking , Invitrogen
code: EI9021.

The recommended voltage for NUPAGE Bis-Tris gels is 200V or 150-175V
for 9 and 17 well gels.

Some good advice in the Manual:
http://tools.invitrogen.com/content/sfs/manuals/nupage_tech_man.pdf


Best,
Stewart


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