Which HRP Grade for Antibody Labeling?

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Fri Apr 30 05:47:20 EST 2010


Dear Experts,

I would like to label an antibody with peroxidase by the periodate
method. Which HRP grade / isoforms / purity should I use? Is there a
practical advantage (or even disadvantage) of "stabilized" peroxidase,
where the amino groups are chemically protected and therefore
oligomerization of the HRP would be prevented?

The AB will be used in westerns and Elisa.

Many thanks!

Wo


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