Methods Digest, Vol 63, Issue 4

novalidaddress from nurfuerspam.de via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Thu Aug 19 03:23:06 EST 2010


Dear Adroit, 

protocol would be exagerating. simpliest method is to freeze (-80 is nice) the slice thoroughly in an eppi, thaw it and spin for 5min at max speed. then use the supernatant.

cheap homemade more quantitative way is: punch a narrow hole in the bottom of a 500µl epi, insert gel slice and freeze. thaw and place small eppi in standard size eppi. spin at moderate speed for 15 min. you may add some TE, soak 10min (or do another freeze) and spin again to increase recovery.

more expensive variant: sigma sells GeneElute Agarose spin columns. place your agarose slice in one of them and spin.

For cleanup, you might do ethanol precipitation or alike

Have fun,

Wo
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