phospho-Akt blotting

Chris McDermott-Roe via (by cjmcdermottroe from
Thu Aug 19 07:38:38 EST 2010

Hi everyone,

Can somebody provide me with a robust blotting protocol for phospho-Akt (in
cardiacmyocytes preferably)?  If you could tell me which Ab and blotting
conditions work best, that would be really excellent!!  Safe to say I'm
tearing my hair out in frustration.

thanks as always


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