phospho-Akt blotting

Chris McDermott-Roe via methods%40net.bio.net (by cjmcdermottroe from googlemail.com)
Thu Aug 19 07:38:38 EST 2010


Hi everyone,

Can somebody provide me with a robust blotting protocol for phospho-Akt (in
cardiacmyocytes preferably)?  If you could tell me which Ab and blotting
conditions work best, that would be really excellent!!  Safe to say I'm
tearing my hair out in frustration.

thanks as always

Chris


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