induction and overexpression of a 7 kDa proteins in E coli
David-Paul Minde
via methods%40net.bio.net
(by davidminde from gmail.com)
Thu Aug 19 14:24:22 EST 2010
... for peptides/miniproteins SMT3 (i.e. yeast "SUMO") fusion can be
extremely useful. Usually, it helps avoiding degradation. Yields up to
some 100 mg pure peptide per liter :=)
have fun,
David Minde (Drs)
Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
Utrecht University
Krytgebouw
Padualaan 8
NL-3584 CH Utrecht
The Netherlands
office phone +31 30 253 4105
(magic:) android +31 652614443
... "Mannschaftsgeist" :)
Science is what happens while we are making other plans (~John Lennon)
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