Polynucleotide 5' Kinase followed by PCR, purification needed?

Jeff Hsu via methods%40net.bio.net (by jeffhsu3 from gmail.com)
Thu Dec 2 10:10:21 EST 2010


I need to 5' phosphorylate some primes prior to PCR.  Do I need to
purify the primers after the kinase reaction or do you think I can
just inactivate the enzyme at 65 degrees C followed by the PCR
reaction?


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