Polynucleotide 5' Kinase followed by PCR, purification needed?

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Thu Dec 2 22:20:28 EST 2010


On Dec 2, 11:10 pm, Jeff Hsu <jeffh... from gmail.com> wrote:
> I need to 5' phosphorylate some primes prior to PCR.  Do I need to
> purify the primers after the kinase reaction or do you think I can
> just inactivate the enzyme at 65 degrees C followed by the PCR
> reaction?

No separate inactivation needed, the initial melting step should kill
PNK.

Wo


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