gel elution of 6KB PCR product

B.Rama chandran via (by chandranbrama from
Sat Dec 25 08:22:35 EST 2010

Dear All,

       I am facing problem in the  gel elution of 6KB PCR product, I am
getting very less yield (~10ng/micto liter). I am using  Sigma gel
extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
that product for restriction digestion. If you have any suggestion please
give me. If you know any other technique which will give better yield please
let me know that.


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