gel elution of 6KB PCR product

B.Rama chandran via methods%40net.bio.net (by chandranbrama from gmail.com)
Sat Dec 25 08:22:35 EST 2010


Dear All,

       I am facing problem in the  gel elution of 6KB PCR product, I am
getting very less yield (~10ng/micto liter). I am using  Sigma gel
extraction kit (catalog no:NA1111). Since yield is very less I couldn't use
that product for restriction digestion. If you have any suggestion please
give me. If you know any other technique which will give better yield please
let me know that.

regards,
B.Ram.


More information about the Methods mailing list