Alternative for PD-10 columns?

Arne K Christensen via methods%40net.bio.net (by arnec from bio.umass.edu)
Tue Feb 2 11:15:17 EST 2010


Depending on your application, you may consider filter concentrators or 
dialysis.

If you're using the columns to desalt or buffer exchange your sample, 
you could use a centrifugation concentrator with a molecular weight 
cutoff just below your protein (or whatever) of interest.  Concentrate, 
then dilute your sample with your new buffer... concentrate, then dilute 
your sample with your new buffer.

Corning Spin-X Ultra Filtration Concentrators

http://www.fisher.co.uk/offers/index.php?pid=354

Alternatively, you could use dialysis membrane, and dialyze your sample 
several times against your new buffer.  Much cheaper.


Nick Theodorakis wrote:
> On Feb 2, 5:26 am, WS <novalidaddr... from nurfuerspam.de> wrote:
>   
>> Dear Experts,
>>
>> is there any commercial (and preferably cheaper) alternative to PD-10
>> desalting columns?
>> (They are filled with Sephadex G-25, sample volume is up to 2.5ml,
>> elution volume is 3.5ml).
>>     
>
>
> Buy the empty disposable columns and fill them yourself.
> Alternatively, if you can work with low (100 ul) volumes, you can pour
> them in 1cc syringes and do spin-column desalting.
>
> Nick
>
> --
> Nick Theodorakis
> nick_theodorakis from hotmail.com
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-- 

__

Arne K Christensen

Postdoctoral Research Associate
University of Massachusetts, Amherst

USGS Conte Anadromous Fish Research Center
One Migratory Way, PO Box 796
Turners Falls, MA  01376

Email: arnec from bio.umass.edu
Phone: (413) 863-3827
  Fax: (413) 863-9810
  URL: www.biobog.com



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