Alternative for PD-10 columns?
watnne from googlemail.com
(by watnne from googlemail.com)
Tue Feb 2 13:42:07 EST 2010
On Tue, 2 Feb 2010 09:51:09 -0800 (PST), WS
<novalidaddress from nurfuerspam.de> wrote:
>Many thanks for your suggestions so far!
>I forgot to mention that (except to my normal behavior in the methods
>+reagents realm where I like to suggest homebrew), this time I am in
>need of a solution that does not require any calibration etc. etc. I
>need reproducibility. I wondered if there was any commercial
>alternative to PD-10, preferably even of the same dimensions (although
>this wouldn't be a problem as long as they are similar scale and have
>a frit on top). I searched the web, of course, but haven't found any
You could also try Ammonium sulfate precipitation of your protein and
then resuspend the pellet in your buffer of choice.
More information about the Methods