Alternative for PD-10 columns?

watnne from via (by watnne from
Tue Feb 2 13:42:07 EST 2010

On Tue, 2 Feb 2010 09:51:09 -0800 (PST), WS
<novalidaddress from> wrote:

>Many thanks for your suggestions so far!
>I forgot to mention that (except to my normal behavior in the methods
>+reagents realm where I like to suggest homebrew), this time I am in
>need of a solution that does not require any calibration etc. etc. I
>need reproducibility. I wondered if there was any commercial
>alternative to PD-10, preferably even of the same dimensions (although
>this wouldn't be a problem as long as they are similar scale and have
>a frit on top). I searched the web, of course, but haven't found any
>alternative. .

You could also try Ammonium sulfate precipitation of your protein and
then resuspend the pellet in your buffer of choice.

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