Dot blot with PVDF

[Aawara Chowdhury]

In <mailman.196.1267199236.25217.methods from net.bio.net>,
 Arne K Christensen <arnec from bio.umass.edu> wrote:

> I have run dot blots with nitrocellulose membrane before; simply add 
> 10ul samples in RSB directly to membrane and wait for them to dry, then 
> block and continue with the Western blot.  However, we only have PVDF 
> membranes on hand right now.  Has anybody here run dot blots on PVDF 
> membrane.  Do you apply the sample to the dry PVDF membrane before the 
> methanol soak?  Will proteins bind to the membrane before the methanol soak?

I have methanol soaked, then soaked in transfer buffer, than 
soaked in TTBS, and used it wet in the dot blotter with very very low
vacuum.

AC
-- 
Email: echo 36434455860060025978157675027927670979097959886449930P | dc