Gel Solubilization Buffer Composition
(by legatekBLAH from hotmail.com)
Sun Jan 10 07:33:55 EST 2010
Jagadish Katam wrote:
> I am working on purifying the DNA by gel extraction procedure and are using the commercially available kits for this purpose. A yellow color gel solubilization buffer is used to dissolve the sliced gel.
> Here i would know the composition of this gel solubilization buffer.
> Any suggestions will be greatly appreciated.
If it's yellow, it's most certainly NaI. Here's the solution I have used
in the past:
90.8 g NaI and 1.5 gm Na2SO3 in 100 ml of water. Filter through 0.45 um
filter and add another 0.5 g Na2SO3. Store in the coldroom in a dark bottle.
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