GST-tagged protein purication from inclusion bodies

Paul J. Phelan via methods%40net.bio.net (by Paul.Phelan from tufts.edu)
Tue Jan 12 10:24:17 EST 2010


Quoting krishn pratap singh <kpsinghniper from gmail.com>:

> Hi,
> Can anyone suggest me a method to purify my GST-tagged proteins from
> inclusion bodies. In which vector should i clone it to get the expression in
> soluble form.
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GE Healthcare have good handbooks on protein purification, including 
one on purifying "Challenging proteins", which includes a section on 
dealing with inclusion bodies - the pdf is here:

http://www1.gelifesciences.com/aptrix/upp00919.nsf/content/LD_367585163-R350



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