GST-tagged protein purication from inclusion bodies
(by davidminde from gmail.com)
Tue Jan 12 11:00:42 EST 2010
??? Do you want soluble or insoluble (e.g. in inclusion bodies).
If soluble just any his-tagged bacterial pET vector could be areasonable
If possible, include some more tags (SUMO, MBP, GST, NusA) in parallel in
your expression test and go for the one with best soluble overexpression in
Also, many proteins don`t solubly express at temperatures above 15 °C ...
and others are always disappearing by degradation unless protected by
coexpression of partner proteins.
David Minde MSc (TUM)
Cellular Protein Chemistry Room 707
Department of Chemistry
Faculty of Science
NL-3584 CH Utrecht
office phone +31 30 253 4105
(magic:) android +31 652614443
Science is what happens while we are making other plans (~John Lennon)
More information about the Methods