Methods Digest, Vol 56, Issue 15-seperating 3.5 and 3.3 kb in agarosegel

Peter Ellis via methods%40net.bio.net (by pjie2 from cam.ac.uk)
Thu Jan 21 04:35:24 EST 2010


Virash Gupta wrote:
 >
> Seperating 3.5 and 3.3 kb in agarosegel.
> [How to do it...]
 >
> Purify DNA and
> clone in a PCR cloning vector and slect clones for right insert.

Given that you will need to clone the DNA and select clones no matter 
what, it may be simpler just to clone the starting mixture, depending on 
the relative intensity of your two bands.

Peter


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