Methods Digest, Vol 56, Issue 15-seperating 3.5 and 3.3 kb in
agarosegel
Peter Ellis
via methods%40net.bio.net
(by pjie2 from cam.ac.uk)
Thu Jan 21 04:35:24 EST 2010
Virash Gupta wrote:
>
> Seperating 3.5 and 3.3 kb in agarosegel.
> [How to do it...]
>
> Purify DNA and
> clone in a PCR cloning vector and slect clones for right insert.
Given that you will need to clone the DNA and select clones no matter
what, it may be simpler just to clone the starting mixture, depending on
the relative intensity of your two bands.
Peter
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