Sodium Borohydride & Gelatin
(by silverstein.joshua from gmail.com)
Thu Jul 15 11:43:34 EST 2010
I would try APTES (3-AMINOPROPYLTRIETHOXYSILANE) coated glass. You can
either make them yourself (there are a lot of protocols available online) or
buy from Electon Microscopy Sciences or Ted Pella. I think the procedure
takes about 3 hours. Honestly I think it's easier to buy them.
Otherwise you can try maybe GE Healthcare's PlusOne bind solution, which is
another silane treatment. I think some people use them for keeping
polyacrylamide gels on glass slides.
Best of luck,
On Thu, Jul 15, 2010 at 12:23 PM, Yvonne Couch
<yvonne.couch from pharm.ox.ac.uk>wrote:
> Hi all,
> I've recently started using sodium borohydride to reduce the
> autofluorescence of my tissue sections. The sections are mounted on
> gelatinized slides and I've noticed that they come away from the slides
> I stain and after running some standard light-based immuno I can only
> conclude this is the borohydride's fault.
> Can anyone suggest a slide coating/type of slide to stop this happening or
> will I have to resort to free floating?
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> Methods from net.bio.net
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