(by christopherfu from hotmail.com)
Fri Jul 23 08:22:07 EST 2010
Would you mind sharing your protocol for making generic home-made electrocompetent cells? I'm currently working with Stratagene's Quikchange XL kit, and I'm obtaining either no or far too few colonies on my agar plates.
Of course there are a whole list of possibilities why the experiment isn't working, but maybe using better competent cells would help.
Do you have a story that started on Hotmail? Tell us now
More information about the Methods