Saponin replacement?

Joshua Silverstein via methods%40net.bio.net (by silverstein.joshua from gmail.com)
Tue Jul 27 10:27:27 EST 2010


Hi Methodologists -

I want to do a Live/Dead Assay on some HUVECs and the protocol calls for
using saponin to kill control cells.  I noticed it is a surfactant, can I
use something like Tween-20 or Triton-X 100 instead that I have around the
lab?  Also what concentration would you use (I usually do 1% Triton to
permeate the cells)?

Thanks all,
Josh


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