anti-phospho S, T & Y antibodies
Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Tue Mar 9 17:07:43 EST 2010
Am 03.03.2010, 08:19 Uhr, schrieb Priscila Peña Diaz <ofiura from gmail.com>:
> Dear All:
> I've been looking recently into phosphorylation of certain membrane
> after a peptide antibody (specific for my protein of interest) fails to
> recognize the protein without prior treatment of the membrane (blotted
> proteins) with phosphatase.
> Most of the phosphorylation assays are usually performed with labelled
> phosphate (32P) but there seem to be antibodies that can also do the job
> I was wondering if anybody has experience with these monoclonal
> S, T & Y antibodies. They look pretty fancy (and expensive) but would
> to hear if anybody has had any actual experience with them.
You can isolate phosphoproteins quite specifically by IMAC with Fe3+ or by
titanium dioxide columns. MS/MS with electron capture dissociation is a
good way to characterise the resulting proteins.
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