Protein precipitation - acetone?

Iraz Toprak Aydin via methods%40net.bio.net (by iraz.aydin from epfl.ch)
Thu Mar 11 10:56:05 EST 2010


Dear all,

 

I have to do a western blot, but my protein concentration is very low, and I
have to run a mini gel. So I was thinking of precipitation the proteins. I
have never done this before. Does acetone have a bad effect on the blotting?
Are there any points that I should be careful about?

 

Thanks in advance...

 

Iraz Toprak Aydin

 

EPFL SV ISREC, Station 19

Batiment SV, SV 2540  

CH-1015 Lausanne

Switzerland

 

Tel: +41 21 693 07 36

 

e-mail:   <mailto:iraz.aydin from epfl.ch> iraz.aydin from epfl.ch

 



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