Protein precipitation - acetone?

[Iraz Toprak Aydin]

Dear all,

 

I have to do a western blot, but my protein concentration is very low, and I
have to run a mini gel. So I was thinking of precipitation the proteins. I
have never done this before. Does acetone have a bad effect on the blotting?
Are there any points that I should be careful about?

 

Thanks in advance...

 

Iraz Toprak Aydin

 

EPFL SV ISREC, Station 19

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