Identifying an E. coli cell line
Pepa Florez Pérez
(by snipeurope from hotmail.com)
Mon Mar 22 11:48:29 EST 2010
Some time ago I used an E. coli cell line (supposed to be some kind of Origami strain) to express a protein.
After having trying different cells, this one was the only where I could clearly see the band corresponding to my protein.
Unfortunately I didn't write down the strain used and it seems that the expression disappears if glycerolates are reused.
Some months later I have tried again to express the protein using different Origami available at my workplace. But I could never reproduce my previous result.
Therefore it would be really useful if I could identify the cells used the first time. Can I do some kind of DNA/RNA protein or other analysis to get this information?
I am becoming desperate since I don't know what else can be done.
Thank you very much,
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