plasmid from non viable glycerol stock

Sunil Dalvi via methods%40net.bio.net (by sgdalvi from gmail.com)
Wed Mar 24 00:51:24 EST 2010


Hello,

I am working with Agrobacterium transformation of sugarcane.I prepared the
glycerol stock of plasmid transformed in E.Coli DH5 alpha and EHA 105. After
some months those are non reviving. What may be the problem? Is there any
method to recover plasmid from glycerol stocks.

Please help me


Is there anybody who can help me

-- 
S.G.Dalvi,


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