at what pH DNA hydrolyzes initially

Dr. Hiranya S. Roychowdhury via methods%40net.bio.net (by hroychow from nmsu.edu)
Thu Mar 25 12:35:27 EST 2010


At even neutral pH if it is not buffered. DNA autocatalysis happens at any
pH below 8, but if the solution is buffered, the pH does not fall during
the process too much to speed up the process. So, indeed, the best way to
preserve nucleic acids is to store them absolutely dry and dessicated. 
Then again, it is quite difficult to re-dissolve too dry samples! Hence, a
compromise is to store in a medium that would prevent loss (DMFA for RNA,
and TE8 for DNA).  DNA may also be stored spot-dried on a cellulosic
support (filter paper) and eluted from there as and when required.


> I would like to know at what acidic pH dose DNA starts hydrolyzing into
> its
> components
>
> --
> With Regards
> uday
> (u.kumar from fz-juelich.de)
> _______________________________________________
> Methods mailing list
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>


-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003



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