Question

Peter Ellis via methods%40net.bio.net (by pjie2 from cam.ac.uk)
Sat Mar 27 18:49:51 EST 2010


Maryam wrote:
> Hi All
> We have 2 genes that are antisense to each other. I was wondering how we can distinguish the expression of these two genes. 
> Thanks in advance.

1)  Strand-specific riboprobes on Northern blot

2)  Specific RT-PCR - you can make it specific by designing the PCR 
based on the intron/exon structure of the forward/reverse transcript. 
Alternatively, if the transcripts are unspliced you can still be 
selective for forward/reverse transcripts by including only one of your 
primers during the reverse transcriptase step.

Peter


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