a question

gholamreza ahmadian via methods%40net.bio.net (by gholamrezaahmadian from yahoo.ca)
Wed May 5 04:37:48 EST 2010


Dear All
I would like to ask a question and I appreciate your any help: 
I am trying to express a betagalactosidase (bgaB) from Geobacillus stearothermophilus in E.coli. bgaB is a thermostable betagalactosidase originally isolated from Geobacillus stearothermophilus. ATCC 7954 , glycosyl hydrolase 42 family. The enzyme catalyses the hydrolysis of terminal, non-reducing terminal beta-D-galactosidase residues. 
It has 3 subunit from N-terminal as : 1- glycosyl hydrolase 42 family domain, -2-non catalytic domain necessary for trimerization and -3- a domain usually found at the C-terminus of beta-galactosidases.
 
I would like to know if cloning just domain 1 (glycosyl hydrolase 42 family domain) is functional or other domain like trimerization domain is also necessary for functional expression of enzyme.
 
Best Regards
Reza



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