Western Blotting

[Yvonne Couch]

Hi all,   

 Quick Western blotting question.in the past my Westerns have all been 'semi

    quantitative' where I use an image programme like ImageJ to calculate
the

    intensity of the different bands.  Apparently it's possible to generate

    standard curves for Western blots, does anyone have any experience of
this

    and can give me some general pointers?

    Thanks in advance

    Yvonne