WS via (by novalidaddress from
Wed May 5 14:17:38 EST 2010

Hi Reza,

trimerization for sure will increase the enzyme's activity. It is hard
to say if the catalytic domain alone will show any activity. This will
a lot depend on where you separate the domains and of course all
domains influence each other. I would not suggest just to place the
cut somewhere and hope that (at least some) activity might remain.

If you are lucky, someone already has done this fragment analysis and
published the results. If not, and you need this information, it will
be up to you to perform these experiments.

hope that helps


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