cellfree/in-vitro-expression of heterodimer

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sun May 16 05:21:14 EST 2010


Hi Jan,

Do you have a possibility to check if you get dimerization at all and
if the subunits are correctly folded? Maybe also some
posttranslational processing is missing (signal sequences, *.ylation
etc etc - any motifs present?). Have you considered to treat your
proteins obtained from the Qiagen kit with eg sf9-cell free extract?

Is there a need for the cell free system or would the expression of
tagged versions of your proteins in your favorite cells, followed by
affinity purification, be an alternative?

Wo


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