Role of maleic acid in S aureus transformation

[yoginee budhkar]

Hello all,

I need to transform S aureus strain with a plasmid of my interest.

In the biorad gene pulser protocol booklet, they mention using SMMP
(sucrose, MgCl2, maleic acid and penassay broth) for reviving the s aureus
after the electroporation step.

I was wondering what is the role of maleic acid in this preparation.

Can it be substituted with anything else?

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Yoginee Budhkar
Contact: +91 9223355677