Role of maleic acid in S aureus transformation

yoginee budhkar via methods%40net.bio.net (by eenigoy from gmail.com)
Mon May 17 05:28:47 EST 2010


Hello all,

I need to transform S aureus strain with a plasmid of my interest.

In the biorad gene pulser protocol booklet, they mention using SMMP
(sucrose, MgCl2, maleic acid and penassay broth) for reviving the s aureus
after the electroporation step.

I was wondering what is the role of maleic acid in this preparation.

Can it be substituted with anything else?

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Yoginee Budhkar
Contact: +91 9223355677


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