how to color the stacking gel?

Ben Thuronyi via (by benthuronyi from
Tue May 18 14:44:53 EST 2010

This page recommends 0.012% bromophenol blue in the upper stacking gel for this purpose, and has photos.

	~ Ben T.

From: "Iraz Toprak Aydin" <iraz.aydin from>
Date: May 18, 2010 3:37:48 AM PDT
To: <methods from>
Subject: how to color the stacking gel?


I have seen some SDS PAGE gels with red  or orange stacking part that makes
the wells visible. They use phenol red for the red stacking gel. But I
couldn't find the concentration of phenol red they use for this purpose.
Does anybody have any experience in this?

Thanks in advance!

Iraz Toprak Aydin

EPFL SV ISREC, Station 19

Batiment SV, SV 2540  

CH-1015 Lausanne


Tel: +41 21 693 07 36

e-mail:   <mailto:iraz.aydin from> iraz.aydin from

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