how to color the stacking gel?

Ben Thuronyi via methods%40net.bio.net (by benthuronyi from berkeley.edu)
Tue May 18 14:44:53 EST 2010


This page recommends 0.012% bromophenol blue in the upper stacking gel for this purpose, and has photos.

http://bitesizebio.com/2010/03/22/sds-page-the-easy-way-to-find-the-wells/

	~ Ben T.

From: "Iraz Toprak Aydin" <iraz.aydin from epfl.ch>
Date: May 18, 2010 3:37:48 AM PDT
To: <methods from magpie.bio.indiana.edu>
Subject: how to color the stacking gel?


Hello!



I have seen some SDS PAGE gels with red  or orange stacking part that makes
the wells visible. They use phenol red for the red stacking gel. But I
couldn't find the concentration of phenol red they use for this purpose.
Does anybody have any experience in this?



Thanks in advance!



Iraz Toprak Aydin



EPFL SV ISREC, Station 19

Batiment SV, SV 2540  

CH-1015 Lausanne

Switzerland



Tel: +41 21 693 07 36



e-mail:   <mailto:iraz.aydin from epfl.ch> iraz.aydin from epfl.ch






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