Methods Digest, Vol 60, Issue 14

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Fri May 28 04:39:02 EST 2010


Align 28S sequences for different fungal species and design primers in
the target region

On Thu, May 27, 2010 at 10:34 PM,  <methods-request from oat.bio.indiana.edu> wrote:
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>   1. Fungal 28s amplification primers (Adroit A)
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> Message: 1
> Date: Thu, 27 May 2010 07:04:08 +0530
> From: Adroit A <cognizann from googlemail.com>
> Subject: Fungal 28s amplification primers
> To: methods from magpie.bio.indiana.edu
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> Dear Bionetters,
>
> I am trying to amplify the 28s rRNA region of a fungus. For this i already
> isolated the genomic DNA and amplified it with ITS-5 and ITS-4 primers. I
> got a PCR product of length approx. 700bp. However this amplified product
> covers the region between 18s-5.8s-28s. Could anyone please suggest me the
> universal primers that specifically amplify the 28s region alone?
>
> I also used ITS-1 and ITS-4 primers for amplification, however no
> amplification was observed. Please let me know the reason for this.
>
> Thanking you in advance.
>
> Thanks,
> Adroit
>
>
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> End of Methods Digest, Vol 60, Issue 14
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-- 
Dr V K Gupta
Sr Microbiologist (Mol Biology)
IMBL, Department of Entomology
Pun. Agric. Univ., Ludhiana (Pb)-141004- India
M: 081465-55515



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