No RNAP but beta-galactosidase

hanna jangg via (by hunnajangg from
Fri Nov 19 10:45:38 EST 2010

Dear Colleagues,
I am currently trying to overexpress RNAP(B.subtilis) in E.coli strain B834 (DE3) pLysS. 
My plasmid DNA(pNG219) contains three of RNAP subunit genes which are downstream of T7 promoter. 
I do fresh transformation but still get very low amount of RNAP(with 9x his-tag). 
Instead of RNAP but Beta-galactosidase is overexpressed. Does anybody have this problem?
I followed protocol for pNG219 that has shown below. 

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